Search results for " Trieste"

Quantification of hydrolysis of toxic organophosphates and organophosphonates by diisopropyl fluorophosphatase from Loligo vulgaris by in situ Fourie…

2008

Abstract The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris effectively catalyzes the hydrolysis of diisopropyl fluorophosphate (DFP) and a number of organophosphorus nerve agents, including sarin, soman, cyclosarin, and tabun. Up to now, the determination of kinetic data has been achieved by techniques such as pH-stat titration, ion-selective electrodes, and fluorogenic substrate analogs. We report a new assaying method using in situ Fourier transform infrared (FTIR) spectroscopy with attenuated total reflection (ATR) for the real-time determination of reaction rates. The method employs changes in the P–O–R stretching vibration of DFP and nerve agent substrate…

Immigrazione femminile in Italia: Trieste e Palermo a confronto

2007

Reversed Enantioselectivity of Diisopropyl Fluorophosphatase against Organophosphorus Nerve Agents by Rational Design

2009

Diisopropyl fluorophosphatase (DFPase) from Loligo vulgaris is an efficient and robust biocatalyst for the hydrolysis of a range of highly toxic organophosphorus compounds including the nerve agents sarin, soman, and cyclosarin. In contrast to the substrate diisopropyl fluorophosphate (DFP) the nerve agents possess an asymmetric phosphorus atom, which leads to pairs of enantiomers that display markedly different toxicities. Wild-type DFPase prefers the less toxic stereoisomers of the substrates which leads to slower detoxification despite rapid hydrolysis. Enzyme engineering efforts based on rational design yielded two quadruple enzyme mutants with reversed enantioselectivity and overall en…

Music Migrations from the Bohemian Lands to Trieste and the National Awakening of the Southern Slavs

2017

After obtaining the status of free port under the Austrian administration (1719), Trieste was transformed into a rich cosmopolitan town of the Adriatic sea, in which conveyed German and Slavic peoples, and then some groups from the Mediterranean countries. Among them, the Czechs played a key role in spreading the classical style at the end of the eighteenth century, as testified by the individual migration of composers to the nearby cities of Gorizia and Ljubljana (František Josef Dusík, Vaclav Vratny, Jan Kejha, Johannes Schreiber). Equally important, in the second part of the nineteenth century, was the creation of the modern violin school thanks to the contribution of Friedrich Pixis’s p…

The Italian “National Opera” Imagined from a Southern Slavic Viewpoint: Franjo Ks. Kuhač and Josip Mandić

2019

The national awakening after the revolutions of 1848, and the related phenomenon of new operatic grammar disseminated from Russia and Bohemia to other countries of Central Europe, were the main factors in promoting a quest of musical autonomy either in Slovenia or in Croatia. In the light of the Southern Slavic people revival, the criticisms on the Italian opera and the Wagnerian Musikdrama, written by the prominent musicologist Franjo Kuhač (1834-1911), and the composer Josip Mandić (1883-1959), reveal two parallel points of view, which have not been taken into account until today. The negative judgement of Kuhač on the last works of Verdi, influenced by Wagner formulae, and the praise of …

Hydrolysis of Phosphotriesters: A Theoretical Analysis of the Enzymatic and Solution Mechanisms

2012

A theoretical study on the alkaline hydrolysis of paraoxon, one of the most popular organophosphorus pesticides, in aqueous solution and in the active site of Pseudomonas diminuta phosphotriesterase (PTE) is presented. Simulations by means of hybrid quantum mechanics/molecular mechanics (QM/MM) potentials show that the hydrolysis of paraoxon takes place through an A(N)D(N) or associative mechanism both in solution and in the active site of PTE. The results correctly reproduce the magnitude of the activation free energies and can be used to rationalize the observed kinetic isotope effects (KIEs) for the hydrolysis of paraoxon in both media. Enzymatic hydrolysis of O,O-diethyl p-chlorophenyl …